Individual Components for Ames Mutagenicity Test Kits
Specific items for microtiter fluctuation assay kits can be ordered separately:
Catalog no. | Description | Storage | Size |
---|---|---|---|
11-406.3L | 30% Mutazyme™ S9 Mix (PB/BNF) for Moltox FT™ Kits | -20°C | 3.25 ml |
26-710.05 | Moltox FT™ Exposure medium | RT | 50 ml |
26-710.1 | Moltox FT™ Exposure medium | RT | 100 ml |
26-710.25 | Moltox FT™ Exposure medium | RT | 250 ml |
26-711.3 | Moltox FT™ Reversion Indicator medium | RT | 300 ml |
26-711.5 | Moltox FT™ Reversion Indicator medium | RT | 500 ml |
26-712.05 | Moltox FT™ Growth medium | RT | 50 ml |
26-712.1 | Moltox FT™ Growth medium | RT | 100 ml |
26-712.25 | Moltox FT™ Growth medium | RT | 250 ml |
26-714.1 | TGA-Culture medium | RT | 100 ml |
26-715 | 10 x TGA-Culture medium | RT | 10 ml |
26-716 | B-Buffer | RT | 35 ml |
26-718 | Stop reagent | RT | 30 ml |
22-147 | Ampicillin | 4°C | 55 mg/vial |
22-148 | ONPG | -20°C | 4.95 mg/vial |
22-149 | 2-Mercaptoethanol | RT | 100 µl |
60-157 | CC 2-Aminoanthracene | 4°C | 100 µg/vial |
60-157.2 | CC 2-Aminoanthracene | 4°C | 2 mg/vial |
60-164 | CC 2-Aminoanthracene | 4°C | 50 µg/vial |
60-158 | CC 9-Aminoacridine-HCl | 4°C | 500 µg/vial |
60-159 | CC 4-Nitroquinoline-N-oxide | 4°C | 50 µg/vial |
60-163 | CC 4-Nitroquinoline-N-oxide | 4°C | 12.5 µg/vial |
60-160 | CC N4-Aminocytidine | 4°C | 2.5 mg/vial |
60-161 | CC 2-Nitrofluorene | 4°C | 50 µg/vial |
71-098.2L | STDisc™ TA98∗, lyoph. | 4°C | 2 discs/vial |
71-100.2L | STDisc™ TA100∗, - lyoph. | 4°C | 2 discs/vial |
71-1535.2L | STDisc™ TA1535∗, lyoph. | 4°C | 2 discs/vial |
71-1537.2L | STDisc™ TA1537∗, lyoph. | 4°C | 2 discs/vial |
72-002.2L | ECDisc™ WP2 trp, pKM101∗, lyoph. | 4°C | 2 discs/vial |
72-003.2L | ECDisc™ WP2 trp, uvrA, pKM101∗, lyoph. | 4°C | 2 discs/vial |
72-188.2L | ECDisc™ WP2 trp, uvrA∗, lyoph. | 4°C | 2 discs/vial |
73-1535psK | PTM™ S. typhimurium TA1535/pSK1002∗for Ames test | -80°C | 1 ml/vial |
32-71001F | FrozenTAMix (strains TA7000 - TA7006)∗ for Ames test | -80°C | 1 ml/vial |
Please note:
In Germany S. typhymurium and E. coli bacterial strains are classified as animal disease pathogens (epizootic pathogens) of risk group 1. Please check for appropriate regulations in your country.
The Salmonella typhimurium strain TA1535/pSK1002∗ for use in the SOS/UMU test is described by Oda et al.1 and offered in Proprietary Transport Medium (PTM™). Salmonella typhimurium TA Mix cells are frozen.
The bacterial strains contained in STDiscs™ and ECDiscs™ are stabilized and lyophilized and for use as culture inoculum. The Salmonella typhimurium strains (TA) are for use in the bacterial mutagenesis assay described by Maron and Ames2. The Escherichia coli strains (WP2 derivatives) are for use in the bacterial mutagenesis assay as described by Green and Muriel3.
All bacterial strains are potential etiologic agents and are intended for use only by those skilled in the safe handling of potentially infectious agents.
∗ According to the German “Gentechnikgesetz” TA1535/pSK1002 (and TA102) have been classified as “genetically modified organism” (GVO/GMO) of risk group 1/BSL1.
1 Oda Y., Nakamura S., Oki I., Kato T., Shinagawa H. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutation Research 147(5):219-29, 1985.
2 Maron D., Ames B. Revised methods for the Salmonella mutagenicity test. Mutation Research 113(3-4):173-215, 1983.
3 Green M.H., Muriel W.J. Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research 38(1):3-32, 1976.