Cell Therapy Media and Accessory
FUJIFILM Irvine Scientific® offers the PRIME-XV® Media Portfolio comprising xeno- and serum-free media for utilization in cell therapy and regenerative medicine.
Validated culture optimized expansion media for T cells, MSCs, Neutral Cells, Amniotic Fluid Stem Cells (AFSCs), Cancer Stem/Initiating Cells or human/murine Hematopoietic Cells (HPCs) and differentiation media for osteogenesis, adipogenesis and chondrogenesis are manufactured under full cGMP conditions and fulfill CE regulations∗. Cell cryopreservation solutions, various substrates and animal-component-free recombinant growth factors complete the portfolio.
PRIME-XV® Media and Accessory for T Cells
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 91146 | PRIME-XV® Dendritic Cell Maturation CDM | 4°C | 500 ml |
| 91154 | PRIME-XV® T Cell CDM | 4°C | 1 l |
| 91141 | PRIME-XV® T Cell Expansion XSFM | 4°C | 1 l |
| 95112 | PRIME-XV® Recombinant Human GM-CSF ACF | -20°C | 20 μg |
| 95117 | PRIME-XV® Recombinant Human TNF-alpha ACF | -20°C | 10 μg |
| 95118 | PRIME-XV® Recombinant Human IL-2 ACF | -20°C | 10 μg |
| 91113 | PRIME-XV® Recombinant Human IL-3 ACF | -20°C | 10 μg |
| 91114 | PRIME-XV® Recombinant Human IL-4 ACF | -20°C | 20 μg |
| 95121 | PRIME-XV® Recombinant Human IL-6 ACF | -20°C | 20 μg |
PRIME-XV® Dendritic Cell Maturation CDM
Chemically-defined, animal component-free medium for cultivation, maturation and maintenance of monocyte-derived dendritic cells for immunogenic applications
- Optimized for differentiation of monocytes into immature dendritic cells (iDCs) and subsequent maturation of mDCs
- Achieve high yields of DCs with the desired characteristics and morphology
- Maintains potency of dendritic cells for T cell stimulation
- Ready-to-use, just supplement with desired cytokine cocktails
- cGMP and chemically-defined - facilitate transfer from research to clinic
First data with this new chemically-defined medium (CDM) are published in a POSTER available in the download area below.
PRIME-XV® T Cell CDM
Chemically-defined, animal component-free medium for T cell culture optimized to deliver consistent growth while maintaining T cell functionality and potency
- Supports vigorous T cell growth in plates, cell culture bags, and in the G-Rex® while maintaining functionality
- Provides lot-to-lot consistency
- Removes the effects of undefined components on T cell phenotypes
- Supports polarization to targeted T cell types such as Th1 and T regulatory cells
- Traceability documentation provided including Certificates of Analysis, Certificates of Origin, and a Drug Master File (DMF) filed with the US FDA
- Extensive QA testing including functionality, sterility, and endotoxin
PRIME-XV® T Cell Expansion XSFM
T cell expansion xeno-free, serum-free medium
- Optimized for the activation and expansion of human T lymphocytes
- Maintains T cell potency
- Supports expansion of memory T cells
- Serum-free and xeno-free formulation reduces risk of contamination and provides better lot-to-lot consistency
- Drug Master File (DMF) registration
- Contains gentamicin
Recombinant Groth Factors / Cytokines
Produced entirely animal-component-free (ACF) in an E.coli expression system. Please inquire for the entire range!
PRIME-XV Dendritic Cell Maturation CDM- Flyer (2017) (PDF, 436 KB)
PRIME-XV T Cell CDM- Poster (2017) (PDF, 1,8 MB)
PRIME-XV T Cell CDM- Flyer (2017) (PDF, 481 KB)
Find more detailed information on the FUJIFILM Irvine Scientific homepage: Dendritic Cells or T Cells
PRIME-XV® Media and Accessory for Mesenchymal Stem/Stroma Cells (MSCs)
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 91135 | PRIME-XV® MSC Expansion SFM | -20°C | 250 ml |
| 91149 | PRIME-XV® MSC Expansion XSFM | -20°C | 250 ml/ 1 l |
| 91132 | PRIME-XV® Osteogenic Differentiation SFM | -20°C | 100 ml |
| 91137 | PRIME-XV® Adipogenic Differentiation SFM | -20°C | 100 ml |
| 91138 | PRIME-XV® Chondrogenic Differentiation XSFM | -20°C | 100 ml |
| 31001 | PRIME-XV® MatrIS F | -20°C | 200 μg |
| 31002 | PRIME-XV® Human Fibronectin | -20°C | 1 mg |
| 31139 | PRIME-XV® FreezIS | -20°C | 10 ml/ 100 ml |
| 91140 | PRIME-XV® FreezIS DMSO-free | -20°C | 10 ml/ 100 ml |
PRIME-XV® MSC Expansion SFM
Human mesenchymal stromal/stem cell (MSC) expansion serum-free medium
- Outperforms leading competitors and serum-containing media in expansion while maintaining MSC characteristics and multipotency
- Supports MSCs derived from bone marrow, adipose tissue and umbilical cord
- Available as a complete, ready-to-use medium
- Requires little to no adaptation from serum-containing medium
PRIME-XV® MSC Expansion XSFM
Human mesenchymal stromal/stem Cell (MSC) expansion xeno-free / serum-free medium
- Maintains MSC quality after extended culture
- Supports MSCs derived from human adipose, human bone marrow, and human umbilical cord
- Xeno-free and serum-free formulation for lot-to-lot consistency
- Drug Master File (DMF) registration
PRIME-XV® Osteogenic Differentiation SFM
Osteogenic differentiation serum-free medium
- Supports robust induction of osteogenesis from human adipose-derived MSCs and human amniotic fluid stem cells (AFSCs)
- Classical staining method and immunocytochemistry demonstrate the presence of osteoblasts and osteocytes
- Available as a complete, ready-to-use medium
PRIME-XV® Adipogenic Differentiation SFM
Adipogenic differentiation serum-free medium
- Supports robust induction of osteogenesis from human adipose-derived MSCs and human amniotic fluid stem cells (AFSCs)
- Classical staining method and immunocytochemistry demonstrate the presence of osteoblasts and osteocytes
- Available as a complete, ready-to-use medium
PRIME-XV® Chondrogenic Differentiation XSFM
Chondrogenic differentiation xeno-free serum-free medium
- Supports robust induction of chondrogenesis from human mesenchymal stem/ stromal cells (MSCs)
- Classical staining method and immunocytochemistry demonstrate the presence of chondrocytes
- Available as a complete, ready-to-use medium
PRIME-XV® Media and Accessory for Neural Cells
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 91201 | PRIME-XV® Neural Basal Medium | 4°C | 500 ml |
| 91131 | PRIME-XV® NPC Expansion XSFM | -20°C | 250 ml |
| 91142 | PRIME-XV® IS21 Supplement (50X) | -20°C | 10 ml |
PRIME-XV® Media for hHPCs, mHPCs, AFSCs and Tumorspheres
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 91211 | PRIME-XV® Hematopoietic Cell Basal XSFM | 4°C | 500 ml |
| 91206 | PRIME-XV® Mouse Hematopoietic Cell Basal Medium | 4°C | 500 ml |
| 91133 | PRIME-XV® AFSC Expansion Medium | -20°C | 250 ml |
| 91130 | PRIME-XV® Tumorsphere SFM | -20°C | 100 ml |
PRIME-XV® Hematopoietic Cell Basal XSFM
Xeno-free, serum-free basal medium for human hematopoietic progenitor cell culture
- Optimized to support vigorous expansion of hematopoietic progenitor cells while maintaining high Total Nucleated Cell (TNC) count and percentages of CD34+, when supplemented with a cocktail of cytokines
- Provides lot-to-lot consistency
- Manufactured to be scalable and to facilitate transfer from research to clinic
- Drug Master File (DMF) registration
PRIME-XV® Attachement Substrates
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 31001 | PRIME-XV® MatrIS F (rHuman Matrix Protein) | -20°C | 200 μg |
| 31002 | PRIME-XV® Human Fibronectin | -20°C | 1 mg |
PRIME-XV® Cell Cryopreservation Solutions
The ability to preserve and thaw primary and stem cells has tremendous potential in allogenic and autologous transplants for clinical therapeutic applications. FUJIFILM Irvine Scientific's cryopreservation products are formulated as chemically-defined, protein-free solutions that are manufactured under GMP.
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| 91139 | PRIME-XV® FreezIS | 4°C | 10 ml/ 100 ml |
| 91140 | PRIME-XV® MSC FreezIS DMSO-free | 4°C | 10 ml/ 100 ml |
PRIME-XV® FreezIS
Protein-free, chemically-defined cryogenic preservation solution for cells and tissues
- Enables preservation of a variety of cell types at -80°C to -196°C environments
- Maintains cell surface marker expression of cells post-thaw
- No additional components are needed
- Drug Master File (DMF) registration
PRIME-XV® MSC FreezIS DMSO-Free
DMSO-free, chemically-defined cryogenic preservation solution for human mesenchymal stem/stromal cells
- Comparable post-thaw cell viability as solutions containing dimethyl sulfoxide (DMSO)
- Maintain potency of mesenchymal stem/stromal cells (MSCs) throughout cryopreservation
- Eliminate risk of DMSO in MSC applications
- Animal component-free, chemically-defined solution
- Enables cell preservation at -80°C to -196°C environments
- Drug Master File (DMF) registration
Downloads
Find more detailed information on the FUJIFILM Irvine Scientific homepage: Cell Therapy Solutions
∗PRIME-XV® products are usually processed under cGMP conditions and for research or further manufacturing use only.
Abbreviations:
ACF ≡ Animal Component-Free
AFSC ≡ Amniotic Fluid Stem Cell
CDM ≡ Chemically Defined Medium
HPC ≡ Hematopoietic Progenitor Cell
MSC ≡ Mesenchymal Stem/Stroma Cell
NPC ≡ Neural Progenitor Cell
SFM ≡ Serum-Free Medium
XSFM ≡ Xeno-Free / Serum-Free Medium
