CellExpand™
Suspension Expansion Culture™ (SEC™) Reagents
For expansion of lympho-hematopoietic cells in suspension
- For 96-well, 24-well, 12-well or 6-well plate suspension cultures or large culture flask volumes
- Easy and rapid high-content screening for proliferation and differentiation processes
- Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process
CellExpand™ is available as master mix for various lympho-hematopoietic cell populations [growth factors contained therein]:
Stem cells
- SC-HPP 1 [IL-3, IL-6, SCF, TPO, Flt3-L]
- SC-HPP 2 [EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L]
- SC-GEMM 1 [EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L]
- SC-GEMM 2 [EPO, GM-CSF, IL-3, IL-6, SCF, TPO]
- SC-GEMM 3 [EPO, GM-CSF, G-CSF, IL-3, IL-6, SCF, TPO]
- SC-GEM 1 [EPO, GM-CSF, IL-3, IL-6, SCF]
- SC-GEM 2 [EPO, GM-CSF, IL-3, SCF]
Progenitor cells
- P-BFU 1 [EPO, IL-3, SCF]
- P-BFU 2 [EPO (high dose)]
- P-GM 1 [GM-CSF, IL-3, SCF]
- P-GM 3 [GM-CSF]
- P-Mk 1 [TPO, IL-3, SCF]
- P-Tcell [IL-2 (also requires addition of co-stimulator)]
- P-Bcell [IL-7]
Use of CellExpand™
- High throughput flow cytometry
- Cell signaling studies
- DNA, RNA analysis and other molecular biology methods
- Mechanism of Action (MOA) studies
- Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process
- Gene transfection and targeting studies, e.g. CRISPR-Cas9
- CellExpand™ Master Mixes are liquid and thereby allow cell interactions providing:
- Shorter lag time to initiate proliferation
- More rapid culture completion
- Greater sensitivity
Specifications
Human (Cat.-No. below), Non-Human Primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)), Canine, Rat or Mouse
Low serum formulations (below) are available for the above listed species in 40 ml or bulk quantities; Serum-free formulations are available for Human, Non-Human Primate and Mouse cells only.
For research use only. Not for clinical diagnostic use.
CellExpand™ Master Mix / SEC™
Catalog no. | Description | Storage | Size |
---|---|---|---|
SEC-0-40H | CellExpand™ without growth factors | -20°C | 40 ml |
SEC-HPP1-40H | CellExpand™ for SC-HPP1 / HPP-SP1 | -20°C | 40 ml |
SEC-HPP2-40H | CellExpand™ for SC- HPP2 / HPP-SP2 | -20°C | 40 ml |
SEC-GEMM1-40H | CellExpand™ for SC-GEMM1 / CFC-GEMM1 | -20°C | 40 ml |
SEC-GEMM2-40H | CellExpand™ for SC-GEMM2 / CFC-GEMM2 | -20°C | 40 ml |
SEC-GEMM3-40H | CellExpand™ for SC- GEMM3 / CFC-GEMM3 | -20°C | 40 ml |
SEC-GEM1-40H | CellExpand™ for SC- GEM1 / CFC-GEM1 | -20°C | 40 ml |
SEC-GEM2-40H | CellExpand™ for SC-GEM2 / CFC-GEM2 | -20°C | 40 ml |
SEC-BFU1-40H | CellExpand™ for P-BFU1 / BFU-E1 | -20°C | 40 ml |
SEC-BFU2-40H | CellExpand™ for P-BFU2 / BFU-E2 | -20°C | 40 ml |
SEC-GM1-40H | CellExpand™ for P-GM1 / GM-CFC1 | -20°C | 40 ml |
SEC-GM3-40H | CellExpand™ for P-GM3 / GM-CFC3 | -20°C | 40 ml |
SEC-Mk1-40H | CellExpand™ for P-Mk1 / MK-CFC1 | -20°C | 40 ml |
SEC-T-40H | CellExpand™ for P-Tcell / T-CFC | -20°C | 40 ml |
SEC-B-40H | CellExpand™ for P-Bcell / B-CFC | -20°C | 40 ml |
This catalog numbers are for Human cells / Low serum formulation; available as well for Primate, Canine, Rat or Mouse. Serum-free formulations are available for Human, Primate and Mouse cells.
Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here