Immune Cell Functional Assays

Whether it is a simple lymphocyte proliferation assay, donor lymphocyte infusion (DLI) testing or mixed cultures, these assays are flexible for any laboratory. Choose from a variety of readouts including ATP bioluminescence, fluorescence and absorbance/colorimetry, and different package sizes.

ImmunoGlo TCP

ATP bioluminescence T-lymphocyte cell proliferation and viability assay kit

  • To measure T-lymphocyte proliferation with a non-radioactive assay
  • T-lymphocyte proliferation assay kits work right out of the box
  • Equal or improved sensitivity to a radioactive marker and 10-100 times increased sensitivity than WST-1 or CSFE assays
  • 3 assay kits available: Growth medium with IL-2, with CD3 + CD28, or with IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
  • A standardized 5-days incubation period can be varied if required
  • Readout has been validated, and can easily re-validated with the included standards and controls

T-lymphocyte proliferation with HemoGro growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo TCP (culture period: 5 days)

Comparison of ImmunoGlo-96 TCP assay kit types
K2-T-1H ImmunoGlo TCP with IL-2 alone -20°C 1 x 96-well kit
KM1-TCS1-1H ImmunoGlo TCP with CD3 + CD28 -20°C 1 x 96-well kit
KM1-TCS2-1H ImmunoGlo TCP with IL-2 + CD3 + CD28 -20°C 1 x 96-well kit

Kit contents:

  • HemoGro Serum-free medium for Human cells containing requested cytokine cocktails
  • ATP Standard
  • ATP Controls
  • ATP Enumeration reagent
  • Sterile, 96-well culture plates for culturing cells
  • Non-sterile, 96-well plates (for assay standardization)
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Assay manual

ImmunoGlo TCP - Flyer (PDF, 470 KB)

ImmunoGlo Real Time

ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls

  • Non-lytic, bioluminescence assay – follow lymphocyte proliferation in real time
  • Multiplexing flow cytometric capability
  • Add ImmunoGlo Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 day
  • Complete flexibility to use any mitogens, co-stimulators etc. and lymphocyte culture protocols
  • For use with peripheral blood mononuclear cells (PBMS), lymphocyte sub-populations and lymphocyte tissues and organs
KM1-RT1 ImmunoGlo Real Time -20°C 1 x 96-well kit

ImmunoGlo - ImmunoFLUOR - ImmunoLIGHT

ATP bioluminescence - fluorescence - absorbance/colorimetric Lymphocyte Proliferation Assays

  • Lymphocyte activation
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies
  • Single-cell, T-lymphocyte cloning studies
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use
  • Effect of accessory (non T-cells) on T-cell induction
  • Effect of co-stimulators on T-cell induction
Catalog no. Description Storage Size
KM1-LPA-1 ImmunoGlo - Bioluminescence lymphocyte prolif. assay -20°C 1 x 96-well kit
KM2-LPA-1 ImmunoFLUOR - Fluorescence lymphoyte prolif. assay -20°C 1 x 96-well kit
KM3-LPA-1 ImmunoLIGHT - Absorbance lymphocyte prolif. assay -20°C 1 x 96-well kit

ImmunoGlo MLC - ImmunoFLUOR MLC - ImmunoLIGHT MLC

ATP bioluminescence - fluorescence - absorbance/colorimetric 1- or 2-way Mixed Lymphocyte Culture assay

  • One-way mixed lymphocyte culture (MLC) / reaction (MLR)
  • Two-way mixed lymphocyte culture (MLC) / reaction (MLR)
  • Allogeneic mixed lymphocyte culture
  • Cellular immune response
  • Dendritic cell interactions
  • Mesenchymal stem/stromal cell interactions
  • Investigations of agents that activate/suppress MLC/MLR
Catalog no. Description Storage Size
KM1-MLC-1 ImmunoGlo MLC - Bioluminescence MLC assay -20°C 1 x 96-well kit
KM2-MLC-1 ImmunoFLUOR MLC - Fluorescence MLC assay -20°C 1 x 96-well kit
KM3-MLC-1 ImmunoLIGHT MLC - Absorbance MLC assay -20°C 1 x 96-well kit