Immune Cell Functional Assays
Whether it is a simple lymphocyte proliferation assay, donor lymphocyte infusion (DLI) testing or mixed cultures, these assays are flexible for any laboratory. Choose from a variety of readouts including ATP bioluminescence, fluorescence and absorbance/colorimetry, and different package sizes.
ImmunoGlo™ TCP
ATP bioluminescence T-lymphocyte cell proliferation and viability assay kit
- To measure T-lymphocyte proliferation with a non-radioactive assay
- T-lymphocyte proliferation assay kits work right out of the box
- Equal or improved sensitivity to a radioactive marker and 10-100 times increased sensitivity than WST-1 or CSFE assays
- 3 assay kits available: Growth medium with IL-2, with CD3 + CD28, or with IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
- A standardized 5-days incubation period can be varied if required
- Readout has been validated, and can easily re-validated with the included standards and controls
T-lymphocyte proliferation with HemoGro™ growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo™ TCP (culture period: 5 days)

CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
K2-T-1H | ImmunoGlo™ TCP with IL-2 alone | -20°C | 1 x 96-well kit |
KM1-TCS1-1H | ImmunoGlo™ TCP with CD3 + CD28 | -20°C | 1 x 96-well kit |
KM1-TCS2-1H | ImmunoGlo™ TCP with IL-2 + CD3 + CD28 | -20°C | 1 x 96-well kit |
Kit contents:
- HemoGro™ Serum-free medium for Human cells containing requested cytokine cocktails
- ATP Standard
- ATP Controls
- ATP Enumeration reagent
- Sterile, 96-well culture plates for culturing cells
- Non-sterile, 96-well plates (for assay standardization)
- Sterile, adhesive foil covers to maintain sterility of unused wells
- Assay manual
ImmunoGlo™ Real Time
ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls
- Non-lytic, bioluminescence assay – follow lymphocyte proliferation in real time
- Multiplexing flow cytometric capability
- Add ImmunoGlo™ Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 day
- Complete flexibility to use any mitogens, co-stimulators etc. and lymphocyte culture protocols
- For use with peripheral blood mononuclear cells (PBMS), lymphocyte sub-populations and lymphocyte tissues and organs
CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
KM1-RT1 | ImmunoGlo™ Real Time | -20°C | 1 x 96-well kit |
ImmunoGlo™ - ImmunoFLUOR™ - ImmunoLIGHT™
ATP bioluminescence - fluorescence - absorbance/colorimetric Lymphocyte Proliferation Assays
- Lymphocyte activation
- Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
- Cellular immune response studies
- Single-cell, T-lymphocyte cloning studies
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use
- Effect of accessory (non T-cells) on T-cell induction
- Effect of co-stimulators on T-cell induction
Catalog no. | Description | Storage | Size |
---|---|---|---|
KM1-LPA-1 | ImmunoGlo™ - Bioluminescence lymphocyte prolif. assay | -20°C | 1 x 96-well kit |
KM2-LPA-1 | ImmunoFLUOR™ - Fluorescence lymphoyte prolif. assay | -20°C | 1 x 96-well kit |
KM3-LPA-1 | ImmunoLIGHT™ - Absorbance lymphocyte prolif. assay | -20°C | 1 x 96-well kit |
ImmunoGlo™ MLC - ImmunoFLUOR™ MLC - ImmunoLIGHT™ MLC
ATP bioluminescence - fluorescence - absorbance/colorimetric 1- or 2-way Mixed Lymphocyte Culture assay
- One-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Two-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Allogeneic mixed lymphocyte culture
- Cellular immune response
- Dendritic cell interactions
- Mesenchymal stem/stromal cell interactions
- Investigations of agents that activate/suppress MLC/MLR
Catalog no. | Description | Storage | Size |
---|---|---|---|
KM1-MLC-1 | ImmunoGlo™ MLC - Bioluminescence MLC assay | -20°C | 1 x 96-well kit |
KM2-MLC-1 | ImmunoFLUOR™ MLC - Fluorescence MLC assay | -20°C | 1 x 96-well kit |
KM3-MLC-1 | ImmunoLIGHT™ MLC - Absorbance MLC assay | -20°C | 1 x 96-well kit |