In vitro Hemotoxicity Testing
The blood-forming or hematopoietic system is a definitive stem cell system and continuously proliferating system in the body. Stem cells and their immediate progenitor cell progeny are extremely sensitive to insults and perturbations. Drugs, compounds and many other agents often affect the hematopoietic system.
HALO®-Tox HT
2-step predictive residual stem cell toxicity and change in drug sensitivity assay
- The only validated, high throughput hemotoxicity assay for in vitro stem and progenitor cells
- HALO®-Tox HT for up to 7 different stem cell populations, 7 lineage-specific hematopoietic progenitor cell populations and 2 lymphopoietic cell populations from 5 species (Human (below), Primate, Canine, Rat and Mouse).
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| K2-0-2HH | HALO®-Tox HT without growth factors | -20°C | 2x 96-well kit |
| K2-HPP2-2HH | HALO®-Tox HT for SC-HPP2 / HPP-SP2 | -20°C | 2x 96-well kit |
| K2-GEMM1-2HH | HALO®-Tox HT for SC-GEMM1 / CFC-GEMM1 | -20°C | 2x 96-well kit |
| K2-B1-2HH | HALO®-Tox HT for P-BFU1 / BFU-E1 | -20°C | 2x 96-well kit |
| K2-GM1-2HH | HALO®-Tox HT for P-GM1 / GM-CFC1 | -20°C | 2x 96-well kit |
| K2-Mk1-2HH | HALO®-Tox HT for P-Mk1 / MK-CFC1 | -20°C | 2x 96-well kit |
| K2-T-2HH | HALO®-Tox HT for P-Tcell / T-CFC | -20°C | 2x 96-well kit |
| K2-B-2HH | HALO®-Tox HT for P-Bcell / B-CFC | -20°C | 2x 96-well kit |
Readout: ATP bioluminescence (96- or 384-well plates)
Kit components: Master mix, ATP standard, ATP controls, 96-well plate(s), sterile adhesive foil, manual
HALO® PRT
ATP bioluminescence predictive residual hemotoxicity assays
When cells respond to cytotoxic agents, not all the cells may be inhibited or killed. The cells that arenot affected are residual cells. When removed and re-plated, these cells may still be capable of proliferation. The residual dose response after secondary re-plating may indicate a shift to the left or right indicating a change in agent/drug sensitivity. Secondary re-plating also indicate the presence of stem cells from that tissue or organ.
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| K2-PRT-2H | HALO® PRT for SC-HPP1 + SC-HPP2, Human | -20°C | 2x 96-well kit |
| K2-PRT-2PR | HALO® PRT for SC-HPP1 + SC-HPP2, Primate (NHP) | -20°C | 2x 96-well kit |
| K2-PRT-2C | HALO® PRT for SC-HPP1 + SC-HPP2, Dog | -20°C | 2x 96-well kit |
| K2-PRT-2R | HALO® PRT for SC-HPP1 + SC-HPP2, Rat | -20°C | 2x 96-well kit |
| K2-PRT-2M | HALO® PRT for SC-HPP1 + SC-HPP2, Mouse | -20°C | 2x 96-well kit |
HALO® PRT is available in "Low serum" (see above) or "Serum-free" (Human, Primate and Mouse) HemoGro™ high-performance culture growth medium
HALO® PMT
"Global" hematopoietic stem and progenitor cell complete assays for 4-, 5- or 7-populations to monitor patient reconstitution after transplantation
- Allows simultaneous detection of hematopoietic stem and progenitor cells to be measured at any time to demonstrate and follow reconstitution
- Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting
- Results are obtained in only 5 days
- Allows comparison of patient results with time due to inclusion of assay standards and to determine "global" reconstitution
- 3 assay formats available:
4-Population assay for SC-GEMM1, P-BFU1, P-GM1 and P-Mk1
5-Population assay for SC-HPP2, SC-GEMM1, P-BFU1, P-GM1 and P-Mk1
7-Population assay for SC-HPP2, SC-GEMM1, P-BFU1, P-GM1, P-Mk1, P-Tcell, P-Bcell
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| K2-4PMT-5 | HALO® PMT for SC-GEMM1, P-BFU1, P-GM1 and P-Mk1, incl. control | -20°C | 5x 96-well kit |
| K2-5PMT-6 | HALO® PMT for SC-HPP2, SC-GEMM1, P-BFU1, P-GM1 and P-Mk1, incl. control | -20°C | 6x 96-well kit |
| K2-7PMT-8 | HALO® PMT for SC-HPP2, SC-GEMM1, P-BFU1, P-GM1, P-Mk1, P-Tcell, P-Bcell, incl. control | -20°C | 8x 96-well kit |
HALO® PMT is available in "Low serum" (see above) or "Serum-free" HemoGro™ high-performance culture growth medium
CAMEO™-96
Clonal, methylcellulose proliferation and differentiation 96-well assays
- Manual colony enumeration AND standardized ATP bioluminescence
- Fast results in 10-12 days: media formulations allows increased growth of colonies
- Economical use of cells
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| KCO-0-1H | CAMEO™-96 without growth factors | -20°C | 1x 96-well kit |
| KCO-HPP1-1H | CAMEO™-96 for SC-HPP1 / HPP-SP1 | -20°C | 1x 96-well kit |
| KCO-HPP2-1H | CAMEO™-96 for SC-HPP2 / HPP-SP2 | -20°C | 1x 96-well kit |
| KCO-GEMM1-1H | CAMEO™-96 for SC-GEMM1 / CFC-GEMM1 | -20°C | 1x 96-well kit |
| KCO-GEMM2-1H | CAMEO™-96 for SC-GEMM2 / CFC-GEMM2 | -20°C | 1x 96-well kit |
| KCO-GEM1-1H | CAMEO™-96 for SC-GEM1 / CFC-GEM1 | -20°C | 1x 96-well kit |
| KCO-GEM2-1H | CAMEO™-96 for SC-GEM2 / CFC-GEM2 | -20°C | 1x 96-well kit |
| KCO-BFU1-1H | CAMEO™-96 for P-BFU1 / BFU-E1 | -20°C | 1x 96-well kit |
| KCO-GM1-1H | CAMEO™-96 for P-GM1 / GM-CFC1 | -20°C | 1x 96-well kit |
| KCO-Mk1-1H | CAMEO™-96 for P-Mk1 / Mk-CFC1 | -20°C | 1x 96-well kit |
| KCO-T-1H | CAMEO™-96 for P-Tcell / T-CFC | -20°C | 1x 96-well kit |
| KCO-B-1H | CAMEO™-96 for P-Bcell / B-CFC | -20°C | 1x 96-well kit |
CAMEO™-4
Miniaturized methylcellulose CFC assay used in toxicity testing
- To detect the response of compounds on the differentiation and maturation process
- Easy handing: due to the optimized blend of the master mix it can simply be applied with a poistive displacement pipette
- Fast results in 10-12 days: media formulations allows increased growth of colonies
- Economical use of cells: the miniaturized CFU-assay kit CAMEO™-4 requires only 1/10 the amount of target cells compared to traditional assays
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| KC-0-50H | CAMEO™-4 without growth factors | -20°C | 38 ml + 50 plates |
| KC-HPP1-50H | CAMEO™-4 for SC-HPP1 / HPP-SP1 | -20°C | 38 ml + 50 plates |
| KC-HPP2-50H | CAMEO™-4 for SC-HPP2 / HPP-SP2 | -20°C | 38 ml + 50 plates |
| KC-GEMM1-50H | CAMEO™-4 for SC-GEMM1 / CFC-GEMM1 | -20°C | 38 ml + 50 plates |
| KC-GEMM2-50H | CAMEO™-4 for SC-GEMM2 / CFC-GEMM2 | -20°C | 38 ml + 50 plates |
| KC-GEMM3-50H | CAMEO™-4 for SC-GEMM3 / CFC-GEMM3 | -20°C | 38 ml + 50 plates |
| KC-GEM1-50H | CAMEO™-4 for SC-GEM1 / CFC-GEM1 | -20°C | 38 ml + 50 plates |
| KC-GEM2-50H | CAMEO™-4 for SC-GEM2 / CFC-GEM2 | -20°C | 38 ml + 50 plates |
| KC-GEM3-50H | CAMEO™-4 for SC-GEM3 / CFC-GEM3 | -20°C | 38 ml + 50 plates |
| KC-BFU1-50H | CAMEO™-4 for P-BFU1 / BFU-E1 | -20°C | 38 ml + 50 plates |
| KC-BFU2-50H | CAMEO™-4 for P-BFU2 / BFU-E2 | -20°C | 38 ml + 50 plates |
| KC-E-50H | CAMEO™-4 for CFU-E | -20°C | 38 ml + 50 plates |
| KC-GM1-50H | CAMEO™-4 for P-GM1 / GM-CFC1 | -20°C | 38 ml + 50 plates |
| KC-GM2-50H | CAMEO™-4 for P-GM2 / GM-CFC2 | -20°C | 38 ml + 50 plates |
| KC-GM3-50H | CAMEO™-4 for P-GM3 / GM-CFC3 | -20°C | 38 ml + 50 plates |
| KC-G-50H | CAMEO™-4 for G-CFC | -20°C | 38 ml + 50 plates |
| KC-M-50H | CAMEO™-4 for M-CFC | -20°C | 38 ml + 50 plates |
| KC-Mk1-50H | CAMEO™-4 for P-Mk1 / Mk-CFC1 | -20°C | 38 ml + 50 plates |
| KC-Mk2-50H | CAMEO™-4 for P-Mk2 / Mk-CFC2 | -20°C | 38 ml + 50 plates |
| KC-T-50H | CAMEO™-4 for P-Tcell / T-CFC | -20°C | 38 ml + 50 plates |
| KC-B-50H | CAMEO™-4 for P-Bcell / B-CFC | -20°C | 38 ml + 50 plates |
Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here
