In vitro Immunotoxicity Testing

Although often considered a separate area, immunotoxicity and hemotoxicity are intimately related since the immune and hematopoietic systems share common primitive stem cells. For the more mature immune cell populations Preferred Cell Systems™ has developed:

ImmunoGlo™ TCP

ATP bioluminescence T-lymphocyte cell proliferation and viability assay kits

  • Non-radioactive assay to measure T-lymphocyte proliferation
  • T-lymphocyte proliferation assay kits which work right out of the box
  • Equal or improved sensitivity to a radioactive marker and 10–100 times increased sensitivity than WST-1 or CSFE assays
  • 3 assay kits available: Growth medium with IL-2, CD3 + CD28, or IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
  • A standardized 5-days incubation period can be varied if required
  • Readout has been validated, and can easily re-validated with the included standards and controls

T-lymphocyte proliferation with HemoGro™ growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo™ TCP (culture period: 5 days)

Comparison of the ImmunoGlo-96 TCP assay kit types

CATALOG NO. DESCRIPTION STORAGE SIZE
K2-T-1H ImmunoGlo™ TCP with IL-2 alone -20°C 1 x 96-well kit
KM1-TCS1-1H ImmunoGlo™ TCP with CD3 + CD28 -20°C 1 x 96-well kit
KM1-TCS2-1H ImmunoGlo™ TCP with IL-2 + CD3 + CD28 -20°C 1 x 96-well kit

Kit contents:

  • HemoGro™ Serum-free medium for Human cells containing requested cytokine cocktails
  • ATP Standard
  • ATP Controls
  • ATP Enumeration reagent
  • Sterile, 96-well culture plates for culturing cells
  • Non-sterile, 96-well plates (for assay standardization)
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Assay manual

ImmunoGlo™ TCP - Flyer (PDF, 470 KB)

ImmunoGlo™-Tox HT

Assays for in vitro immunotoxicity

This standardized ATP bioluminescence 96- or 384-well plate in vitro immunotoxicity testing system can be used to detect cytotoxicity associated with stimulator and responder cells.

CATALOG NO. DESCRIPTION STORAGE SIZE
KM1-T96-2 ImmunoGlo™-Tox HT for user defined immune cells -20°C 2 x 96-well kit
KM1-T96-4 ImmunoGlo™-Tox HT for user defined immune cells -20°C 4 x 96-well kit
KM1-T384-2 ImmunoGlo™-Tox HT for user defined immune cells -20°C 2 x 384-well kit
KM1-T384-4 ImmunoGlo™-Tox HT for user defined immune cells -20°C 4 x 384-well kit
KM1-T96CS1-2H ImmunoGlo™-Tox HT with CD3 + CD28, Human -20°C 2 x 96-well kit
KM1-T384CS1-2H ImmunoGlo™-Tox HT with CD3 + CD28, Human -20°C 2 x 384-well kit
KM1-T96CS2-2H ImmunoGlo™-Tox HT with IL-2 + CD3 + CD28, Human -20°C 2 x 96-well kit
KM1-T384CS2-2H ImmunoGlo™-Tox HT with IL-2 + CD3 + CD28, Human -20°C 2 x 384-well kit

ImmunoGlo™ Real Time

ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls

  • Non-lytic, bioluminescence assay – follow lymphocyte proliferation in real time
  • Multiplexing flow cytometric capability
  • Add ImmunoGlo™ Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 day
  • Complete flexibility to use any mitogens, co-stimulators etc. and lymphocyte culture protocols
  • For use with peripheral blood mononuclear cells (PBMS), lymphocyte sub-populations and lymphocyte tissues and organs
CATALOG NO. DESCRIPTION STORAGE SIZE
KM1-RT1 ImmunoGlo™ Real Time -20°C 1 x 96-well kit