In vitro Immunotoxicity Testing
Although often considered a separate area, immunotoxicity and hemotoxicity are intimately related since the immune and hematopoietic systems share common primitive stem cells. For the more mature immune cell populations Preferred Cell Systems™ has developed:
ImmunoGlo™ TCP
ATP bioluminescence T-lymphocyte cell proliferation and viability assay kits
- Non-radioactive assay to measure T-lymphocyte proliferation
- T-lymphocyte proliferation assay kits which work right out of the box
- Equal or improved sensitivity to a radioactive marker and 10–100 times increased sensitivity than WST-1 or CSFE assays
- 3 assay kits available: Growth medium with IL-2, CD3 + CD28, or IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
- A standardized 5-days incubation period can be varied if required
- Readout has been validated, and can easily re-validated with the included standards and controls
T-lymphocyte proliferation with HemoGro™ growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo™ TCP (culture period: 5 days)
CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
K2-T-1H | ImmunoGlo™ TCP with IL-2 alone | -20°C | 1 x 96-well kit |
KM1-TCS1-1H | ImmunoGlo™ TCP with CD3 + CD28 | -20°C | 1 x 96-well kit |
KM1-TCS2-1H | ImmunoGlo™ TCP with IL-2 + CD3 + CD28 | -20°C | 1 x 96-well kit |
Kit contents:
- HemoGro™ Serum-free medium for Human cells containing requested cytokine cocktails
- ATP Standard
- ATP Controls
- ATP Enumeration reagent
- Sterile, 96-well culture plates for culturing cells
- Non-sterile, 96-well plates (for assay standardization)
- Sterile, adhesive foil covers to maintain sterility of unused wells
- Assay manual
ImmunoGlo™-Tox HT
Assays for in vitro immunotoxicity
This standardized ATP bioluminescence 96- or 384-well plate in vitro immunotoxicity testing system can be used to detect cytotoxicity associated with stimulator and responder cells.
CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
KM1-T96-2 | ImmunoGlo™-Tox HT for user defined immune cells | -20°C | 2 x 96-well kit |
KM1-T96-4 | ImmunoGlo™-Tox HT for user defined immune cells | -20°C | 4 x 96-well kit |
KM1-T384-2 | ImmunoGlo™-Tox HT for user defined immune cells | -20°C | 2 x 384-well kit |
KM1-T384-4 | ImmunoGlo™-Tox HT for user defined immune cells | -20°C | 4 x 384-well kit |
KM1-T96CS1-2H | ImmunoGlo™-Tox HT with CD3 + CD28, Human | -20°C | 2 x 96-well kit |
KM1-T384CS1-2H | ImmunoGlo™-Tox HT with CD3 + CD28, Human | -20°C | 2 x 384-well kit |
KM1-T96CS2-2H | ImmunoGlo™-Tox HT with IL-2 + CD3 + CD28, Human | -20°C | 2 x 96-well kit |
KM1-T384CS2-2H | ImmunoGlo™-Tox HT with IL-2 + CD3 + CD28, Human | -20°C | 2 x 384-well kit |
ImmunoGlo™ Real Time
ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls
- Non-lytic, bioluminescence assay – follow lymphocyte proliferation in real time
- Multiplexing flow cytometric capability
- Add ImmunoGlo™ Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 day
- Complete flexibility to use any mitogens, co-stimulators etc. and lymphocyte culture protocols
- For use with peripheral blood mononuclear cells (PBMS), lymphocyte sub-populations and lymphocyte tissues and organs
CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
KM1-RT1 | ImmunoGlo™ Real Time | -20°C | 1 x 96-well kit |