In vitro Mesenchymal Stem Cell Toxicity Testing System
The production of osteoblasts, adipocytes and chondrocytes play a large role in regenerative medicine. These cell types are all derived from mesenchymal stem cells (MSCs). Mesenchymal stem cells are also part of the hematopoietic stroma of the hematopoietic stem cell niche.
MSCGlo™-Tox HT
Assays for in vitro MSC toxicity
- MSC bioluminescence toxicity platform utilizing bioluminescence technology
- Assays include standards and controls to calibrate and standardize
CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
---|---|---|---|
KLMC-T96CR-2H KLMC-T96CR-4H |
MSCGlo™-Tox HT with CRUX RUFA Human Platelet Lysate | -20°C | 2 x 96-well kit 4 x 96-well kit |
KLMC-T96LS-2H KLMC-T96LS-4H |
MSCGlo™-Tox HT with Low serum medium | -20°C | 2 x 96-well kit 4 x 96-well kit |
KLMC-T96SF-2H KLMC-T96SF-4H |
MSCGlo™-Tox HT with Serum-free medium | -20°C | 2 x 96-well kit 4 x 96-well kit |
KLMC-T96H-2H KLMC-T96H-4H |
MSCGlo™-Tox HT with Humanized medium | -20°C | 2 x 96-well kit 4 x 96-well kit |
MSCGlo™ PRT
MSC predictive residual toxicity assays
When cells respond to cytotoxic agents, not all the cells may be inhibited or killed. The cells that are not affected are residual cells. When removed and re-plated, these cells may still be capable of proliferation. The residual dose response after secondary re-plating may indicate a shift to the left or right indicating a change in agent/drug sensitivity. Secondary re-plating also indicate the presence of stem cells from that tissue or organ.
Catalog no. | Description | Storage | Size |
---|---|---|---|
KLMC-PRTCR-2 | MSCGlo™ PRT with CRUX RUFA Human Platelet Lysate | -20°C | 2 x 96-well kit |
KLMC-PRTLS-2 | MSCGlo™ PRT with Low serum medium | -20°C | 2 x 96-well kit |
KLMC-PRTSF-2 | MSCGlo™ PRT with Serum-free medium | -20°C | 2 x 96-well kit |
MSCGlo™ Real Time
Real time, non-lytic bioluminescence assay to monitor cell processing of mesenchymal stroma cells
- Watch MSCs grow over 2-3 days by taking bioluminescence readings at any times you like
- Non-lytic, bioluminescence assay; thus compatible with downstream applications:
e.g. flow cytometric analysis with fluorochrome-conjugated antibodies (CD73+, CD90+, CD105+ and/or CD106+) to observe changes in phenotypic markers with time - MSCGlo™ RealTime includes your choice of medium:
- CRUX RUFA Human Platelet Lysate
- MSCGro Low serum complete medium for Human, Primate, Rat or Mouse
- MSCGro Serum-free, xeno-free complete medium for Human, Primate, Rat or Mouse
- MSCGro Humanized complete medium
- MSCGro medium (Low serum or Serum-free) for Canine or for Equine MSCs on request
Catalog no. | Description | Storage | Size |
---|---|---|---|
KMRT-CR-1 | MSCGlo™ RealTime with CRUX RUFA | -20°C | 1 x 96 well kit |
KMRT-LS-1 | MSCGlo™ RealTime with MSCGro Low serum | -20°C | 1 x 96-well kit |
KMRT-SF-1 | MSCGlo™ RealTime with MSCGro Serum-free | -20°C | 1 x 96-well kit |
KMRT-H-1 | MSCGlo™ RealTime with MSCGro Humanized | -20°C | 1 x 96-well kit |