Instrument-based Proliferation Assays
Instead of detecting differentiation, CFU-equivalent assays directly measure cell proliferation of hematopoietic stem and progenitor cells. Proliferation occurs prior to differentiation and therefore results from CFU-equivalent assays are obtained already after 5-7 days instead of 10-14 days.
All our CFU-equivalent assays incorporate Suspension Expansion Culture (SEC™) technology instead of methylcellulose. They are all instrument-based using either a bioluminescence, fluorescence or absorbance readout. SEC™ technology significantly reduces dispensing errors associated with methylcellulose, but also allows cell interactions to occur that results in shorter lag times to initiate proliferation.
Principle Bioluminescence:
HALO® PCA
ATP bioluminescence CFU equivalent assay
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| K2-PCA1-1 | HALO® PCA for SC-GEM2 / CFC-GEM2 | -20°C | 1 x 96-well kit |
| K2-PCA2-1 | HALO® PCA for SC-GEM3 / CFC-GEM3 | -20°C | 1 x 96-well kit |
| K2-PCA3-1 | HALO® PCA for P-GM1 / CFC-GM1 | -20°C | 1 x 96-well kit |
| K2-PCA4-1 | HALO® PCA for P-GM2 / CFC-GM2 | -20°C | 1 x 96-well kit |
| K2-PCA5-1 | HALO® PCA for SC-GEMM3 / CFC-GEMM3 | -20°C | 1 x 96-well kit |
HALO® PCA is available in "Low serum" (see above) or "Serum-free" HemoGro™ high-performance culture growth medium, and in different package sizes.
HemoFLUOR™ PCA
Fluorescence CFU equivalent assay
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| K5-PCA1-1 | HemoFLUOR™ PCA for SC-GEM2 / CFC-GEM2 | -20°C | 1 x 96-well kit |
| K5-PCA2-1 | HemoFLUOR™ PCA for SC-GEM3 / CFC-GEM3 | -20°C | 1 x 96-well kit |
| K5-PCA3-1 | HemoFLUOR™ PCA for P-GM1 / CFC-GM1 | -20°C | 1 x 96-well kit |
| K5-PCA4-1 | HemoFLUOR™ PCA for P-GM2 / CFC-GM2 | -20°C | 1 x 96-well kit |
| K5-PCA5-1 | HemoFLUOR™ PCA for SC-GEMM3 / CFC-GEMM3 | -20°C | 1 x 96-well kit |
HemoFLUOR™ PCA is available in "Low serum" (see above) or "Serum-free" HemoGro™ high-performance culture growth medium, and in different package sizes.
HemoLIGHT™ PCA
Absorbance/colorimetric CFU equivalent assay
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| K4-PCA1-1 | HemoLIGHT™ PCA for SC-GEM2 / CFC-GEM2 | -20°C | 1 x 96-well kit |
| K4-PCA2-1 | HemoLIGHT™ PCA for SC-GEM3 / CFC-GEM3 | -20°C | 1 x 96-well kit |
| K4-PCA3-1 | HemoLIGHT™ PCA for P-GM1 / CFC-GM1 | -20°C | 1 x 96-well kit |
| K4-PCA4-1 | HemoLIGHT™ PCA for P-GM2 / CFC-GM2 | -20°C | 1 x 96-well kit |
| K4-PCA5-1 | HemoLIGHT™ PCA for SC-GEMM3 / CFC-GEMM3 | -20°C | 1 x 96-well kit |
HemoLIGHT™ PCA is available in "Low serum" (see above) or "Serum-free" HemoGro™ high-performance culture growth medium, and in different package sizes.
HALO® Real Time
Real time, non-lytic bioluminescence assay to monitor cell processing of hematopoietic stem/progenitor cells
- Non-lytic, bioluminescence assay. Compatible with downstream applications, e.g flow cytometric phenotypic analysis (e.g. CD133+, CD34+ or ALDH+)
- Watch the cells grow over 2-5 days by taking bioluminescence readings at any times you like
- For human cells, bone marrow, umbilical cord blood normal or mobilized peripheral blood or purified populations (e.g. CD34+, CD133+)
- Available for 7 lympho-hematopoietic stem cell and 9 progenitor cell populations
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| K6-HPP1-1H | HALO® Real Time for SC-HPP1 / HPP-SP1 | -20°C | 1 x 96-well kit |
| K6-HPP2-1H | HALO® Real Time for SC-HPP2 / HPP-SP2 | -20°C | 1 x 96-well kit |
| K6-GEMM1-1H | HALO® Real Time for SC-GEMM1 / CFC-GEMM1 | -20°C | 1 x 96-well kit |
| K6-GEMM2-1H | HALO® Real Time for SC-GEMM2 / CFC-GEMM2 | -20°C | 1 x 96-well kit |
| K6-GEM1-1H | HALO® Real Time for SC-GEM1 / CFC-GEM1 | -20°C | 1 x 96-well kit |
| K6-GEM2-1H | HALO® Real Time for SC-GEM2 / CFC-GEM2 | -20°C | 1 x 96-well kit |
| K6-BFU1-1H | HALO® Real Time for P-BFU1 / BFU-E1 | -20°C | 1 x 96-well kit |
| K6-BFU2-1H | HALO® Real Time for P-BFU2 / BFU-E2 | -20°C | 1 x 96-well kit |
| K6-GM1-1H | HALO® Real Time for P-GM1 / GM-CFC1 | -20°C | 1 x 96-well kit |
| K6-GM3-1H | HALO® Real Time for P-GM3 / GM-CFC3 | -20°C | 1 x 96-well kit |
| K6-Mk1-1H | HALO® Real Time for P-Mk1 / Mk-CFC1 | -20°C | 1 x 96-well kit |
HALO® Real Time is available either "Serum-free", or "Low serum" (see above) combined with the following species: Human (see above), Primate, Rat, Mouse and Canine.
Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here
