Mesenchymal Stem/Stromal Cell (MSC) Assays and Reagents

Use a quantitative MSC proliferation assay to characterize MSC-derived, regenerative medicine products. Bioluminescence assays (MSCGlo), fluorescence assays (MCSFluor) and absorbance/colorimetric assays (MCSLight) are available.

  • Monitor small, medium and large/bulk MSC expansion (passaging)
  • For replacement of the manual CFU-F assays
  • Multiplex with other assays such as flow cytometry for MSC and differentiation markers and gene expression analysis to obtain and correlate information from a single sample
  • MSCGlo assays include your choice of medium:
    • CRUX RUFA Human Platelet Lysate
    • MSCGro Low serum complete medium for Human, Primate, Rat or Mouse
    • MSCGro Serum-free, xeno-free complete medium for Human, Primate, Rat or Mouse
    • MSCGro Humanized complete medium
    • MSCGro medium (Low serum or Serum-free) for Canine and Equine MSCs on request

MSCGlo

ATP bioluminescence MSC proliferation assay

Catalog no. Description Storage Size
KLMC-CR-1 MSCGlo, CRUX RUFA -20°C 1 x 96-well kit
KLMC-LS-1 MSCGlo, Low serum -20°C 1 x 96-well kit
KLMC-SF-1 MSCGlo, Serum-free -20°C 1 x 96-well kit
KLMC-HM-1 MSCGlo, Humanized -20°C 1 x 96-well kit

The MSCGlo assay includes standards and controls to calibrate and standardize and, if necessary, validate.


MSCFluor

Fluorescence MSC proliferation assay

Catalog no. Description Storage Size
KMCF-CR-1 MSCFluor, CRUX RUFA -20°C 1 x 96-well kit
KMCF-LS-1 MSCFluor, Low serum -20°C 1 x 96-well kit
KMCF-SF-1 MSCFluor, Serum-free -20°C 1 x 96-well kit
KMCF-HM-1 MSCFluor, Humanized -20°C 1 x 96-well kit

MSCLight

Absorbance / colorimetric MSC proliferation assay

Catalog no. Description Storage Size
KMCL-CR-1 MSCLight, CRUX RUFA -20°C 1 x 96-well kit
KMCL-LS-1 MSCLight, Low serum -20°C 1 x 96-well kit
KMCL-SF-1 MSCLight, Serum-free -20°C 1 x 96-well kit
KMCL-HM-1 MSCLight, Humanized -20°C 1 x 96-well kit

MSCGlo Real Time

Real time, non-lytic bioluminescence assay to monitor cell processing of mesenchymal stroma cells

  • Watch MSCs grow over 2-3 days by taking bioluminescence readings at any times you like
  • Non-lytic, bioluminescence assay; thus compatible with downstream applications: e.g. flow cytometric analysis with fluorochrome-conjugated antibodies (CD73+, CD90+, CD105+ and/or CD106+) to observe changes in phenotypic markers with time
  • Choose your most suitable MSC Medium: MSCGro medium or CRUX RUFA Human Platelet Lysate
Catalog no. Description Storage Size
KMRT-CR-1 MSCGlo Real Time, CRUX RUFA -20°C 1 x 96-well kit
KMRT-LS-1 MSCGlo Real Time, Low serum -20°C 1 x 96-well kit
KMRT-SF-1 MSCGlo Real Time, Serum-free -20°C 1 x 96-well kit
KMRT-H-1 MSCGlo Real Time, Humanized -20°C 1 x 96-well kit

MSCGlo Real Time - Flyer (PDF, 434 KB)