Mesenchymal Stroma Cell (MSC) Assays

For regenerative medicine applications, the "quality" and potency of the MSCs are of paramount importance. MSC "quality" is measured by proliferation ability, while potency is measured by proliferation potential, two properties that help define und characterize stem cells. Use a quantitative MSC proliferation assay to characterize an MSC-derived, regenerative medicine product.

MSCGlo™ assays include your choice of medium:

  • CRUX RUFA Human Platelet Lysate
  • MSCGro Low serum complete medium for Human, Primate, Rat or Mouse
  • MSCGro Serum-free, xeno-free complete medium for Human, Primate, Rat or Mouse
  • MSCGro Humanized complete medium
  • MSCGro medium (Low serum or Serum-free) for Canine or for Equine MSCs on request

MSCGlo™ QC

ATP bioluminescence MSC quality assays to monitor cell processing, cryopreservation and purification methods

  • MSCGlo™ QC (MSCGlo™-96 HuQC) determines "quality", that is the ability of mesenchymal stem/stromal cells to proliferate
  • Instrument-based, incorporating an external standard and controls for assay standardization and validation
  • Compare results over time for intra- and inter-laboratory studies
  • Assay can usually be completed in 5-7 days of culture depending on the batch of MSCs
Catalog no. Description Storage Size
KLMC-CRQC-1 MSCGlo™ QC, CRUX RUFA -20°C 1 x 96-well kit
KLMC-LSQC-1 MSCGlo™ QC, Low serum -20°C 1 x 96-well kit
KLMC-SFQC-1 MSCGlo™ QC, Serum-free -20°C 1 x 96-well kit
KLMC-HMQC-1 MSCGlo™ QC, Humanized -20°C 1 x 96-well kit

MSCGlo™ SC-IPS/Potency

ATP bioluminescence assays for in-house RS + MSC samples to determine Stem Cell Identity, Purity and Strength (SC-IPS)

  • MSCGlo™ SC-IPS/Potency (PQR) is the only reference standard-based potency assay for mesenchymal stem/stromal cells commercially available
  • MSCGlo™ SC-IPS/Potency is based on the same proven principles of measuring potency of lympho-hematopoietic stem cells as HALO® SC-IPS/Potency
  • Includes additional standards and controls which, together with the reference standard, allows the assay to be validated
Catalog no. Description Storage Size
KLMC-CRP-1

MSCGlo™ SC-IPS, CRUX RUFA

 -20°C 1 x 96-well kit
KLMC-LSP-1 MSCGlo™ SC-IPS, Low serum -20°C 1 x 96-well kit
KLMC-SFP-1 MSCGlo™ SC-IPS, Serum-free -20°C 1 x 96-well kit
KLMC-HMP-1 MSCGlo™ SC-IPS, Humanized -20°C 1 x 96-well kit
MSCGlo™ SC-IPS/Potency and RS - Flyer (PDF, 887 KB)

MSCGlo™ RS

ATP bioluminescence reference standard-based potency assays for MSC/MPC samples

  • Contains cryopreserved reference standard cells to establish an in-house RS (reference standard)
  • Standardized and validated ATP-bioluminescence assay to establish a cell dose-response curve to determine the proliferation potential
  • Instrument based assay readout
Catalog no. Description Storage Size
KLMC-CRRS-1CB* MSCGlo™ RS, CRUX RUFA -20°C 1 x 96-well kit
KLMC-LSRS-1CB*

MSCGlo™ RS, Low serum

 -20°C 1 x 96-well kit
KLMC-SFRS-1CB* MSCGlo™ RS, Serum-free -20°C 1 x 96-well kit
KLMC-HMRS-1CB* MSCGlo™ RS, Humanized -20°C 1 x 96-well kit
KLMC-CRRS-1BM* MSCGlo™ RS, CRUX RUFA -20°C 1 x 96-well kit
KLMC-LSRS-1BM*

MSCGlo™ RS, Low serum

 -20°C 1 x 96-well kit
KLMC-SFRS-1BM* MSCGlo™ RS, Serum-free -20°C 1 x 96-well kit
KLMC-HMRS-1BM* MSCGlo™ RS, Humanized -20°C 1 x 96-well kit

* CB = for MSCs from Cord Blood; BM = for MSCs from Bone Marrow

MSCGlo™ SC-IPS/Potency and RS - Flyer (PDF, 887 KB)

MSCGlo™ Real Time

Real time, non-lytic bioluminescence assay to monitor cell processing of mesenchymal stroma cells

  • Watch MSCs grow over 2-3 days by taking bioluminescence readings at any times you like
  • Use a non-lytic, bioluminescence assay; thus compatible with downstream applications:
    e.g flow cytometric analysis with fluorochrome-conjugated antibodies (CD73+, CD90+, CD105+ and/or CD106+) to observe changes in phenotypic markers with time
  • Choose your most suitable MSC medium: MSCGro medium or CRUX RUFA Human Platelet Lysate
Catalog no. Description Storage Size
KMRT-CR-1 MSCGlo™ Real Time, CRUX RUFA -20°C 1 x 96 well kit
KMRT-LS-1 MSCGlo™ Real Time, Low serum -20°C 1 x 96-well kit
KMRT-SF-1 MSCGlo™ Real Time, Serum-free -20°C 1 x 96-well kit
KMRT-H-1 MSCGlo™ Real Time, Humanized -20°C 1 x 96-well kit
MSCGlo™ Real Time - Flyer (PDF, 434 KB)