Individual Components for Ames Mutagenicity Test Kits

Ames test components

Specific items for microtiter fluctuation assay kits can be ordered separately:

Catalog no. Description Storage Size
11-406.3L 30% Mutazyme S9 Mix (PB/BNF) for Moltox FT Kits -20°C 3.25 ml
26-710.05 Moltox FT Exposure medium RT 50 ml
26-710.1 Moltox FT Exposure medium RT 100 ml
26-710.25 Moltox FT Exposure medium RT 250 ml
26-711.3 Moltox FT Reversion Indicator medium RT 300 ml
26-711.5 Moltox FT Reversion Indicator medium RT 500 ml
26-712.05 Moltox FT Growth medium RT 50 ml
26-712.1 Moltox FT Growth medium RT 100 ml
26-712.25 Moltox FT Growth medium RT 250 ml
26-714.1 TGA-Culture medium RT 100 ml
26-715 10 x TGA-Culture medium RT 10 ml
26-716 B-Buffer RT 35 ml
26-718 Stop reagent RT 30 ml
22-147 Ampicillin 4°C 55 mg/vial
22-148 ONPG -20°C 4.95 mg/vial
22-149 2-Mercaptoethanol RT 100 µl
60-157 CC 2-Aminoanthracene 4°C 100 µg/vial
60-157.2 CC 2-Aminoanthracene 4°C 2 mg/vial
60-164 CC 2-Aminoanthracene 4°C 50 µg/vial
60-158 CC 9-Aminoacridine-HCl 4°C 500 µg/vial
60-159 CC 4-Nitroquinoline-N-oxide 4°C 50 µg/vial
60-163 CC 4-Nitroquinoline-N-oxide 4°C 12.5 µg/vial
60-160 CC N4-Aminocytidine 4°C 2.5 mg/vial
60-161 CC 2-Nitrofluorene 4°C 50 µg/vial
71-098.2L STDisc TA98, lyoph. 4°C 2 discs/vial
71-100.2L STDisc TA100, - lyoph. 4°C 2 discs/vial
71-1535.2L STDisc TA1535, lyoph. 4°C 2 discs/vial
71-1537.2L STDisc TA1537, lyoph. 4°C 2 discs/vial
72-002.2L ECDisc WP2 trp, pKM101, lyoph. 4°C 2 discs/vial
72-003.2L ECDisc WP2 trp, uvrA, pKM101, lyoph. 4°C 2 discs/vial
72-188.2L ECDisc WP2 trp, uvrA, lyoph. 4°C 2 discs/vial
73-1535psK PTM S. typhimurium TA1535/pSK1002for Ames test -80°C 1 ml/vial
32-71001F FrozenTAMix (strains TA7000 - TA7006) for Ames test -80°C 1 ml/vial

Please note:
In Germany S. typhymurium and E. coli bacterial strains are classified as animal disease pathogens (epizootic pathogens) of risk group 1. Please check for appropriate regulations in your country.

The Salmonella typhimurium strain TA1535/pSK1002 for use in the SOS/UMU test is described by Oda et al.1 and offered in Proprietary Transport Medium (PTM). Salmonella typhimurium TA Mix cells are frozen.
The bacterial strains contained in STDiscs and ECDiscs are stabilized and lyophilized and for use as culture inoculum. The Salmonella typhimurium strains (TA) are for use in the bacterial mutagenesis assay described by Maron and Ames2. The Escherichia coli strains (WP2 derivatives) are for use in the bacterial mutagenesis assay as described by Green and Muriel3.
All bacterial strains are potential etiologic agents and are intended for use only by those skilled in the safe handling of potentially infectious agents.

According to the German “Gentechnikgesetz” TA1535/pSK1002 (and TA102) have been classified as “genetically modified organism” (GVO/GMO) of risk group 1/BSL1.

1 Oda Y., Nakamura S., Oki I., Kato T., Shinagawa H. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutation Research 147(5):219-29, 1985.
2 Maron D., Ames B. Revised methods for the Salmonella mutagenicity test. Mutation Research 113(3-4):173-215, 1983.
3 Green M.H., Muriel W.J. Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research 38(1):3-32, 1976.