Regensys™ NADPH Regenerating System
NADP/NADPH is a necessary cofactor in various metabolism reactions. NADP is added to S9 liver extract to support the oxidase activity catalyzed by P450s, FMOs, NADPH-P450 reductase, and many other oxidase enzymes.
The Moltox® NADPH Regenerating System consists of two reagents: Regensys™ A and Regensys™ B and is intended for preparation of a 4-10% S9 mix, as described by Maron and Ames1. The fill sizes refer to final volume of S9 mix per package.
- Content Regensys™ A: Gucose-6-phosphate + MgCl2/KCl in 0.1M Phosphate buffer, pH7.4
- Content Regensys™ B: lyophilized NADP
Regensys™ Components
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| 60-200.15 | NADPH Regensys A | 4°C | 15 ml |
| 60-200.40 | NADPH Regensys A | 4°C | 40 ml |
| 60-200.5 | NADPH Regensys A | 4°C | 50 ml |
| 60-201.15L | NADPH Regensys B, lyoph. | -20°C | 46 mg |
| 60-201.4L | NADPH Regensys B, lyoph. | -20°C | 123 mg |
| 60-201.5L | NADPH Regensys B, lyoph. | -20°C | 153 mg |
| 60-203.1 | Glucose-6-phosphate | RT | 2 g |
Our ready-to-use S9 Mix MUTAZYME™ already contains Regensys™ A and Regensys™ B.
1 Maron D., Ames B. Revised methods for the Salmonella mutagenicity test. Mutation Research 113(3-4):173-215, 1983.
