Microtiter Fluctuation Assay Kits

Moltox FT™ TA98/TA100 Mutagenicity Assay Kit

Intended to perform a Ames microtiter fluctuation test using Salmonella typhimurium strains TA98 and TA100

The kit contents is sufficient to perform triplicate analyses of 6 concentrations of the test material, positive and negative controls (with and without S9):

  • STDiscs™ TA98 and TA100 (2 discs per strain/vial)
  • FT™ Growth medium, FT™ Exposure medium, FT™ Reversion Indicator medium
  • Ampicillin, 55 mg/vial
  • MUTAZYME™ Moltox FT™ 30% S9 Mix, Aroclor induced rat liver, 3.25 ml, lyophilized
  • Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, 2-Nitrofluorene
  • User instructions
Catalog no. Description Storage Size
31-300 Moltox FT™ TA98 & TA100 Mutagenicity Assay Kit - 1 sample kit
31-300- Instruction Manual

Moltox FT™ 471 Mutagenicity Assay Kit

Moltox FT assay components

Intended to perform a Ames microtiter fluctuation test using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and an Escherichia coli WP2 strain

The kit contents is sufficient to perform triplicate analyses of 6 concentrations of the test material, positive and negative controls (with and without S9). Consistent with OECD 4713:

  • STDiscs™ TA98, TA100, TA1535, TA1537 (2x 2 discs/vial each) and ECDisc™ WP2 trp, uvrA (2x 2 discs/vial)
  • FT™ Growth medium, FT™ Exposure medium, FT™ Reversion Indicator medium (2x 500 ml)
  • Ampicillin, 55 mg/vial
  • MUTAZYME™ 30% S9 Mix, Aroclor induced rat liver, 6.5 ml, lyophilized
  • Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, N4-aminocytidine, 2-Nitrofluorene, 9-Aminoacridine
  • User instruction
Catalog no. Description Storage Size
31-301 Moltox FT™ 471 Mutagenicity Assay Kit - 1 sample kit
31-301- Instruction Manual

Moltox FT™ E. coli Mutagenicity Assay Kit

Intended to perform a Ames microtiter fluctuation test using Escherichia coli WP2 strains

The use of these Escherichia coli strains in the bacterial mutagenesis assay was described by Green and Muriel1.

The kit contents is sufficient to test 1 sample in triplicate (or 3 samples with no replicate), +/- S9 at various concentrations (≤6), positive controls and solvent (negative control):

  • ECDiscs™ WP2 trp, pKM101, WP2 trp, uvrA (2 discs/vial each)
  • FT™ Growth medium, FT™ Exposure medium, FT™ Reversion Indicator medium
  • Ampicillin, 55 mg/vial
  • MUTAZYME™ 30% S9 Mix, Aroclor induced rat liver, 3.25 ml, lyophilized
  • Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
  • User instruction
Catalog no. Description Storage Size
31-302 Moltox FT™ E. coli Mutagenicity Assay Kit - 1 sample kit
31-302- Instruction Manual

Ames II™ Mutagenicity Assay Kit by BioReliance®

BioReliance Logo AmesII assay components

Intended to perform a microtiter fluctuation test using Salmonella typhimurium TA98 and TAMix

The mutations arising due to frame shift events are detected by TA98. TAMix consists of a mixture of TA7000 through TA7006 strains responsive to each of the several possible base-pair substitution events.

The reagents provided in this kit are sufficient to test 1 sample in triplicate (or 3 samples with no replicate), +/- S9 at various concentrations (≤6), positive controls and solvent (negative control):

  • STDiscs™ TA98 /(2 discs/vial) and TAMix (1ml/vial)
  • Growth media, Exposure media, Indicator media
  • Ampicillin, sterile
  • MUTAZYME™ 30% S9 Mix for Ames II™ Kits, Aroclor induced rat liver, lyophilized
  • Positive controls: 2-Nitrofluorene, 4-Nitroquinoline-N-oxide, 2-Aminoanthracene
  • Software disc and instruction manual
Catalog no. Description Storage Size
32-102 Ames II™ Mutagenicity Assay Kit by BioReliance® - 1 sample kit
32-102- Instruction Manual

UMU Genotoxicity Test Kit

UMU assay components

Suitable to perform the UMU genotoxicity assay as described by Reifferscheid et al.2 and in accordance with ISO 13829

The reagents provided in this kit are sufficient to test 3 test materials with no replicates or 1 sample in triplicate, +/- S9 at various concentrations (≤6), positive controls and solvent (negative control).

  • PTM™ Salmonella typhimurium TA1535/pSK1002∗ (2vials)
  • TGA Culture medium, Ampicillin, B-Buffer, Stop Reagent, ONPG, 2-Mercaptoethanol
  • MUTAZYME™ 30% S9 Mix, Aroclor induced rat liver, 3.25 ml, lyophilized
  • Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
  • User instruction
Catalog no. Description Storage Size
31-400∗ Moltox UMU Genotoxicity Test Kit - 1 sample kit
31-400∗- Product Information (PDF, 546 KB)

31-400∗- Instruction Manual

∗ According to the German “Gentechnikgesetz”, TA1535/pSK1002 has been classified as “genetically modified organism” (GVO/GMO) of risk group 1/BSL1. It is offered in proprietary transport medium (PTM™). Please check for appropriate regulations in your country.

1 Green, M.H.L. and Muriel, W.J. Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research, 38(1):3-32, 1976.
2 Reifferscheid, G. et al. A microplate version of the SOS/umu-test for rapid detection of genotoxins and genotoxic potentials of environmental samples. Mutation Research, 253(3):215-22, 1991.
3 OECD Guideline 471 for the Testing of Chemicals, Section 4. Test No. 471: Bacterial Reverse Mutation Test