Microtiter Fluctuation Assay Kits

Moltox FT^™ TA98/TA100 Mutagenicity Test Kit

Intended to perform a Ames microtiter fluctuation test using Salmonella typhimurium strains TA98 and TA100

The kit contents is sufficient to perform triplicate analyses of 6 concentrations of the test material, positive and negative controls (with and without S9):

• STDiscs^™ TA98 and TA100 (2 discs/vial each)
• FT^™ Growth medium, FT^™ Exposure medium, FT^™ Reversion Indicator medium
• Ampicillin, 55 mg/vial
• 30% MUTAZYME^™ S9 Mix for Moltox FT^™, 3.25 ml, lyophilized
• Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, 2-Nitrofluorene
• Instruction Manual

Moltox FT^™ 471 Mutagenicity Test Kit

Intended to perform a Ames microtiter fluctuation test using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and an Escherichia coli WP2 strain

The kit contents is sufficient to perform triplicate analyses of 6 concentrations of the test material, positive and negative controls (with and without S9). Consistent with OECD 471³:

• STDiscs^™ TA98, TA100, TA1535, TA1537 and ECDisc^™ WP2 trp, uvrA (2 discs/vial each)
• FT^™ Growth medium, FT^™ Exposure medium, FT^™ Reversion Indicator medium (2x 500 ml)
• Ampicillin, 55 mg/vial
• 30% MUTAZYME^™ S9 Mix for Moltox FT^™, 3.25 ml, lyophilized
• Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, N4-Aminocytidine, 2-Nitrofluorene, 9-Aminoacridine-HCl
• Instruction Manual

Moltox FT^™ E. coli Mutagenicity Test Kit

Intended to perform a Ames microtiter fluctuation test using Escherichia coli WP2 strains

The use of these Escherichia coli strains in the bacterial mutagenesis assay was described by Green and Muriel¹.

The kit contents is sufficient to test 1 sample in triplicate (or 3 samples with no replicate), +/- S9 at various concentrations (≤6), positive controls and solvent (negative control):

• ECDiscs^™ WP2 trp, pKM101 and WP2 trp, uvrA (2 discs/vial each)
• FT^™ Growth medium, FT^™ Exposure medium, FT^™ Reversion Indicator medium
• Ampicillin, 55 mg/vial
• 30% MUTAZYME^™ S9 Mix for Moltox FT^™, 3.25 ml, lyophilized
• Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
• Instruction Manual

UMU Genotoxicity Test Kit

Suitable to perform the UMU genotoxicity assay as described by Reifferscheid et al.² and in accordance with ISO 13829

The reagents provided in this kit are sufficient to test 3 test materials with no replicates or 1 sample in triplicate, +/- S9 at various concentrations (≤6), positive controls and solvent (negative control):

• PTM^™ Salmonella typhimurium TA1535/pSK1002^{∗ 4} (2 vials)
• TGA Culture medium, Ampicillin, B-buffer, Stop Reagent, ONPG, 2-Mercaptoethanol
• 30% MUTAZYME^™ S9 Mix for Moltox FT^™, 3.25 ml, lyophilized
• Positive controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
• Instruction manual

^∗ According to the German “Gentechnikgesetz”, TA1535/pSK1002 has been classified as “genetically modified organism” (GVO/GMO) of risk group 1/BSL1. It is offered in proprietary transport medium (PTM^™). Please check for appropriate regulations in your country.

¹ Green, M.H.L. and Muriel, W.J. Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research, 38(1):3-32, 1976.
² Reifferscheid, G. et al. A microplate version of the SOS/umu-test for rapid detection of genotoxins and genotoxic potentials of environmental samples. Mutation Research, 253(3):215-22, 1991.
³ OECD Guideline 471 for the Testing of Chemicals, Section 4. Test No. 471: Bacterial Reverse Mutation Test
⁴ Oda Y., Nakamura S., Oki I., Kato T., Shinagawa H. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutation Research 147(5):219-29, 1985.
Matsushima T., Sawamura M., Hara K. and Sugimura T. A Safe Substitute for Polychlorinated Biphenyls as an Inducer of Metabolic Activation Systems. In: "In vitro Metabolic Activation in Mutagenesis Testing", Eds F.J. de Serres et al. Elsevier, North Holland, pp. 85-88, 1976.